Mary Wang's MSc Defence
Butyrate is a short-chain fatty acid with considerable benefits to gut health, and in recent years has been commonly used as an alternative to antimicrobials in agricultural animal production. In this thesis, we assessed the protective effects of sodium butyrate (NaB) on larval zebrafish during a lethal Pseudomonas aeruginosa polysaccharide (LPS) challenge and elucidated potential protective mechanisms of action. Larval zebrafish were pre-treated with 3 concen-trations of NaB for 24 hours at 72 hours post fertilization (hpf), and then immune challenged with 60 μg/mL of LPS at 96 hpf.